2VZZ

Crystal structure of Rv0802c from Mycobacterium tuberculosis in Complex with Succinyl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2004-03-16
Detector	RIGAKU IMAGE PLATE
Spacegroup name	I 2 2 2
Unit cell lengths	113.165, 135.172, 164.077
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	104.260 - 2.300
R-factor	0.172
Rwork	0.169
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vzy
RMSD bond length	0.015
RMSD bond angle	1.684
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.060	0.200
Number of reflections	53759
<I/σ(I)>	15.3	4.8
Completeness [%]	95.9	92.8
Redundancy	3.5	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		PROTEIN AT 8 MG/ML IN 20 MM TEA PH 8.5, 100 MM AMMONIUM SULFATE, 1 MM EDTA, 1 MM DTT, 2 MM COA, 4 MM SUCCOA CRYSTALLIZED IN 1 M LICL, 100 MM HEPES PH 7.0. CRYOPROTECTED IN PRECIPITANT SOLUTION PLUS 20 MM SUCCOA AND 20% GLYCEROL