2VZV

Substrate Complex of Amycolatopsis orientalis exo-chitosanase CsxA E541A with chitosan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	113
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	86.860, 121.410, 184.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	185.700 - 2.700
R-factor	0.186
Rwork	0.182
R-free	0.25500
Structure solution method	OTHER
RMSD bond length	0.010
RMSD bond angle	1.282
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.700
Rmerge	0.120
Number of reflections	47221
<I/σ(I)>	15.4
Completeness [%]	91.8
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1