2VZU
Complex of Amycolatopsis orientalis exo-chitosanase CsxA D469A with PNP-beta-D-glucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.600, 121.960, 91.810 |
| Unit cell angles | 90.00, 90.42, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.100 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.696 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.120 |
| Number of reflections | 105174 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 99.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | mother liquor containing 18% polyethylene glycol 2000 monomethylether, 20 mM CdSO4, and 100 mM Tris-HCl, pH 7.5, |
