2VZT
Complex of Amycolatopsis orientalis exo-chitosanase CsxA E541A with PNP-beta-D-glucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.690, 122.230, 91.860 |
| Unit cell angles | 90.00, 90.52, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.200 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.21800 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.645 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.150 |
| Number of reflections | 91212 |
| <I/σ(I)> | 9.8 |
| Completeness [%] | 99.0 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | mother liquor containing 18% polyethylene glycol 2000 monomethylether, 20 mM CdSO4, and 100 mM Tris-HCl, pH 7.5, |
