2VZO

Crystal structure of Amycolatopsis orientalis exo-chitosanase CsxA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	113
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	86.400, 122.035, 91.602
Unit cell angles	90.00, 90.52, 90.00

Refinement procedure

Resolution	20.000 - 2.240
R-factor	0.178
Rwork	0.175
R-free	0.22300
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.017
RMSD bond angle	1.665
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.240
Rmerge	0.110
Number of reflections	85561
<I/σ(I)>	18.7
Completeness [%]	99.3
Redundancy	19.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1