2VZL
FERREDOXIN-NADP REDUCTASE (MUTATIONS: T155G, A160T, L263P AND Y303S) COMPLEXED WITH NAD BY COCRYSTALLIZATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER-NONIUS |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2007-12-12 |
| Detector | BRUKER-NONIUS |
| Spacegroup name | P 65 |
| Unit cell lengths | 86.888, 86.888, 96.372 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.930 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1que |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.440 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.120 | 0.470 |
| Number of reflections | 31094 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 8.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 20% PEG 6000 0.1M ACNA, PH 5.5 B-OCTYL GLYCOSIDE AT 2%(W/V) 10-60 MM NAD |
