2VZD
Crystal structure of the C-terminal calponin homology domain of alpha parvin in complex with paxillin LD1 motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-05 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.123, 37.503, 70.244 |
| Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
| Resolution | 28.640 - 2.100 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vzc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.030 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 20260 | |
| <I/σ(I)> | 16.4 | 2.5 |
| Completeness [%] | 98.5 | 99.4 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% (W/V) PEG 10000, 0.1M HEPES PH 7.5 |
