2VZC
Crystal structure of the C-terminal calponin homology domain of alpha parvin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.144, 71.197, 47.146 |
| Unit cell angles | 90.00, 99.88, 90.00 |
Refinement procedure
| Resolution | 46.420 - 1.050 |
| R-factor | 0.144 |
| Rwork | 0.143 |
| R-free | 0.15900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1WKU 1tjt 2eyi 1mb8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.690 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.740 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.040 | 0.460 |
| Number of reflections | 124910 | |
| <I/σ(I)> | 16.5 | 2.4 |
| Completeness [%] | 93.7 | 77.7 |
| Redundancy | 4.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 12% (W/V) PEG 8000, 35% (V/V) MPD, 0.1M HEPES PH 7.5 |
