2VYZ
Mutant Ala55Phe of Cerebratulus lacteus mini-hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.880, 43.352, 59.883 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.550 - 1.800 |
| R-factor | 0.19276 |
| Rwork | 0.188 |
| R-free | 0.23003 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kr7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.245 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.360 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.120 | 0.280 |
| Number of reflections | 10801 | |
| <I/σ(I)> | 9.5 | 4.1 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | 60% AMMONIUM SULFATE, 50MM SODIUM ACETATE, PH 6.0, AT 277K |
