2VYW
Hemoglobin (Hb2) from trematode Fasciola hepatica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.677, 59.604, 42.626 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 34.610 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h97 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.148 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.700 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.280 |
| Number of reflections | 12291 | |
| <I/σ(I)> | 21.8 | 5.7 |
| Completeness [%] | 99.0 | 94.7 |
| Redundancy | 7.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | 30% PEG 4000, 0.2M MG-CHLORIDE, 0.1M NA-CACODYLATE PH 7.0, 0.2M NA-THIOCYANATE AT 277K. |
