2VYE
Crystal Structure of the DnaC-ssDNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 180.830, 180.830, 104.112 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.940 - 4.100 |
| R-factor | 0.302 |
| Rwork | 0.302 |
| R-free | 0.37400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vyf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.250 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.060 | 0.470 |
| Number of reflections | 14077 | |
| <I/σ(I)> | 34.3 | 2.4 |
| Completeness [%] | 91.1 | 91.5 |
| Redundancy | 6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
