2VY6
Two domains from the C-terminal region of influenza A virus polymerase PB2 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.380, 65.350, 45.540 |
| Unit cell angles | 90.00, 91.99, 90.00 |
Refinement procedure
| Resolution | 26.470 - 1.950 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 627-DOMAIN FROM PDB ENTRY 2VY7 AND NLS DOMAIN FROM PDB ENTRY 2JDQ |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.583 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.130 | 0.570 |
| Number of reflections | 14078 | |
| <I/σ(I)> | 8.95 | 2.28 |
| Completeness [%] | 98.3 | 92.9 |
| Redundancy | 4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1 MICROLITRE OF 5.5 MG/ML PROTEIN SOLUTION IN 10 MM TRIS-HCL PH 7.0 AND 200 MM NACL WITH EQUAL VOLUME OF 100 MM HEPES PH 7.5 AND K/NA TARTRATE AT 1.2 M. |
