2VY3
Type IV secretion system effector protein BepA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.336, 84.610, 126.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.219 |
Rwork | 0.216 |
R-free | 0.27600 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.019 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.130 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | 0.320 |
Number of reflections | 18356 | |
<I/σ(I)> | 6.25 | 2.37 |
Completeness [%] | 91.6 | 60.9 |
Redundancy | 5.56 | 2.58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.7 | 17 MG/ML PROTEIN IN 20 MM TRIS-HCL (PH 8.0), 500 MM NACL AND 2 MM BETA-MERCAPTOETHANOL MIXED WITH 22 % POLYETHYLENE GLYCOL 4000, 0.1 M TRIS PH 8.7, 5 MM NICL2 IN A 1:1 (V:V) RATIO |