2VY2
Structure of LEAFY transcription factor from Arabidopsis thaliana in complex with DNA from AG-I promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-07-20 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.850, 98.850, 177.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.951 - 2.300 |
| R-factor | 0.2315 |
| Rwork | 0.230 |
| R-free | 0.26190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vy1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.394 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.750 |
| Number of reflections | 328932 | |
| <I/σ(I)> | 26.7 | 3.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10% PEG 400, 100 MM KCL, 10 MM CACL2, 50 MM HEPES (NAOH) PH 7.0 |
