2VXX
X-ray structure of DpsA from Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-22 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 174.504, 174.504, 174.504 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 123.090 - 2.400 |
| R-factor | 0.16739 |
| Rwork | 0.165 |
| R-free | 0.21567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1moj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.987 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0043) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.180 | 0.730 |
| Number of reflections | 34749 | |
| <I/σ(I)> | 10.86 | 2.53 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.3 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% OF PEG 8000, 0.1M OF MES, PH 6.0 |
