2VXJ
CRYSTAL STRUCTURE OF PA-IL LECTIN COMPLEXED WITH AGAL13BGAL14GLC AT 1.9 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.184, 86.521, 119.078 |
| Unit cell angles | 93.93, 98.16, 90.08 |
Refinement procedure
| Resolution | 55.000 - 1.900 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l7l |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.568 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.220 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.360 |
| Number of reflections | 230909 | |
| <I/σ(I)> | 5.45 | 2.08 |
| Completeness [%] | 93.9 | 89.1 |
| Redundancy | 2.26 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 10% PEG 5000 MME, 0.025M KSCN, 0.1M NAAC PH 4.6 |
