2VXI
The binding of heme and zinc in Escherichia coli Bacterioferritin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.739, 1.729 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 208.100, 208.100, 142.768 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 147.440 - 1.910 |
| R-factor | 0.17925 |
| Rwork | 0.179 |
| R-free | 0.21561 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.704 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.380 |
| Number of reflections | 460546 | |
| <I/σ(I)> | 11 | 2.14 |
| Completeness [%] | 97.7 | 90.2 |
| Redundancy | 3.77 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 60% AMSO4, 20MM TRIS-HCL PH7.5, 0.1M NACL |
