2VXH
The crystal structure of chlorite dismutase: a detox enzyme producing molecular oxygen
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-15 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.98340, 1.73990, 1.73820 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 164.458, 169.335, 60.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.680 - 2.100 |
| R-factor | 0.2205 |
| Rwork | 0.219 |
| R-free | 0.25486 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.298 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CRANK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.130 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.410 |
| Number of reflections | 100003 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.8 | 14.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 100 MM MES PH 5.5, 25 %(W/V) PEG MME 2000, 0.3 M KSCN, 5 %(V/V) GLYCEROL, 180 MM AMMONIUM SULPHATE |
