2VX8
Vamp7 longin domain Hrb peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.514, 115.102, 55.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.710 - 2.200 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.28000 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.996 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.290 |
| Number of reflections | 34876 | |
| <I/σ(I)> | 22.7 | 6.6 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 20% PEG 6000, 100MM NA CITRATE PH 4.0, 0.2M LICL, FLASH COOLED IN 22%PEG, 20% GLYCEROL |
