2VX6

CELLVIBRIO JAPONICUS MANNANASE CJMAN26C Gal1Man4-BOUND FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 61 2 2
Unit cell lengths	84.529, 84.529, 244.322
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.530 - 1.570
R-factor	0.11002
Rwork	0.109
R-free	0.13672
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j9y
RMSD bond length	0.011
RMSD bond angle	1.410
Phasing software	MOLREP
Refinement software	REFMAC (5.4.0077)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.630
High resolution limit [Å]	1.570	1.570
Rmerge	0.060	0.250
Number of reflections	72691
<I/σ(I)>	38.4	10.4
Completeness [%]	99.4	99.3
Redundancy	11.3	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1