2VX4

CELLVIBRIO JAPONICUS MANNANASE CJMAN26C NATIVE FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	52.273, 83.606, 84.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.620 - 1.700
R-factor	0.15014
Rwork	0.148
R-free	0.18814
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j9y
RMSD bond length	0.010
RMSD bond angle	1.231
Phasing software	MOLREP
Refinement software	REFMAC (5.4.0076)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.080	0.490
Number of reflections	41464
<I/σ(I)>	19.4	3.7
Completeness [%]	100.0	100
Redundancy	7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1