2VWJ
Uracil Recognition in Archaeal DNA Polymerases Captured by X-ray Crystallography.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 100.700, 161.910, 221.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.730 - 2.780 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tgo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.407 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 45193 | |
| <I/σ(I)> | 11.1 | 2.2 |
| Completeness [%] | 98.7 | 98.9 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.3 | 0.2M TRIPOTASSIUM CITRATE PH8.3, 20% PEG 3350 |
