2VWG
Haloferax mediterranei glucose dehydrogenase in complex with NADP, Zn and gluconolactone.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-18 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.647, 112.205, 150.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.136 |
| Rwork | 0.135 |
| R-free | 0.15900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b5v |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.222 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.360 |
| Number of reflections | 35315 | |
| <I/σ(I)> | 17.4 | 2.6 |
| Completeness [%] | 98.9 | 99.2 |
| Redundancy | 7.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.4M SODIUM CITRATE, HEPES PH7.5. |
