2VWD
Nipah Virus Attachment Glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 77.2 |
| Detector technology | CCD |
| Collection date | 2007-08-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.633, 86.936, 82.903 |
| Unit cell angles | 90.00, 108.32, 90.00 |
Refinement procedure
| Resolution | 39.940 - 2.250 |
| R-factor | 0.175 |
| Rwork | 0.172 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vsm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.209 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.140 | 0.870 |
| Number of reflections | 45810 | |
| <I/σ(I)> | 18.4 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 20% (V/V) PEG 6000, 0.1 M MES PH 6.0, 0.1 M LICL AND 0.1 M GAMMA-BUTYLACTONE 18% |
