2VW8
Crystal Structure of Quinolone signal response protein pqsE from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.370, 57.690, 50.240 |
| Unit cell angles | 90.00, 97.07, 90.00 |
Refinement procedure
| Resolution | 51.300 - 1.450 |
| R-factor | 0.16713 |
| Rwork | 0.166 |
| R-free | 0.18597 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.173 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.040 | 0.420 |
| Number of reflections | 51128 | |
| <I/σ(I)> | 18.39 | 3.11 |
| Completeness [%] | 97.9 | 94.9 |
| Redundancy | 3.78 | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 40% PEG 400, 0.1M NA-CACODYLATE, PH6.0, 0.2M MAGNESIUM ACETATE |
