2VW7

Nitrite reductase from Alcaligenes xylosoxidans - 1 of 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	89.072, 89.072, 288.268
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.240 - 1.900
R-factor	0.164
Rwork	0.162
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	10E1
RMSD bond length	0.011
RMSD bond angle	1.298
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.039	0.124
Number of reflections	66937
<I/σ(I)>	20.3	3.8
Completeness [%]	97.7
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		pH 6.5