2VVO
Crystal structure of Mycobacterium tuberculosis ribose-5-phosphate isomerase B in complex with alpha d-allose 6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-03-18 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.744, 92.501, 60.967 |
| Unit cell angles | 90.00, 98.92, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.850 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1usl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.441 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.190 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.070 | 0.510 |
| Number of reflections | 65360 | |
| <I/σ(I)> | 12.8 | 2.6 |
| Completeness [%] | 91.7 | 61.8 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1M TRI-NA CITRATE, 0.1M NA CACODYLATE PH 6.5 |
