2VUY
Crystal structure of Glycogen Debranching exzyme TreX from Sulfolobus solfatarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2006-02-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 203.633, 203.633, 89.428 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.860 - 3.000 |
| Rwork | 0.206 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bf2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Phasing software | PHASER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.240 |
| Number of reflections | 39857 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 98.4 | 95.8 |
| Redundancy | 10.5 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 16% PEG 8000, 0.2M NACL, 0.1 CHESS BUFFER(PH9.5) |
