2VUO
Crystal structure of the rabbit IgG Fc fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.662, 71.211, 69.036 |
| Unit cell angles | 90.00, 104.78, 90.00 |
Refinement procedure
| Resolution | 56.721 - 1.950 |
| R-factor | 0.1676 |
| Rwork | 0.169 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h3t |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.938 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.700 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.110 | 0.450 |
| Number of reflections | 40194 | |
| <I/σ(I)> | 17.2 | 3.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.3 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 291 | HANGING DROP, VAPOUR DIFFUSION AT 291K. 2M SODIUM FORMATE, 0.1M SODIUM ACETATE, PH 4.7, PROTEIN CONCENTRATION 2MG/ML |
