2VUI
Crystal structure of the HupR receiver domain in inhibitory phospho- state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 81.893, 81.893, 61.812 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.300 - 2.900 |
| R-factor | 0.2609 |
| Rwork | 0.258 |
| R-free | 0.32020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jk1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.252 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.300 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.050 | 0.320 |
| Number of reflections | 5316 | |
| <I/σ(I)> | 27.3 | 5 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.4 M NACL, 0.2 M MGCL2, 0.1 M TRIS-HCL PH 8.0, 25% GLYCEROL, 7 MM BECL2, 29 MM NAF |
