2VUH
Crystal structure of the D55E mutant of the HupR receiver domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 81.782, 81.782, 61.046 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.520 - 2.500 |
| R-factor | 0.22204 |
| Rwork | 0.221 |
| R-free | 0.24714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jk1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.763 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.520 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.340 |
| Number of reflections | 7797 | |
| <I/σ(I)> | 26.5 | 6.4 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 8.1 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.4 M NACL, 0.2 M MGCL2, 0.1 M TRIS-HCL PH 8.0, 28% ETHYLENE GLYCOL |
