2VUC
PA-IIL lectin from Pseudomonas aeruginosa complexed with Fucose- derived glycomimetics
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2006-04-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.555, 72.668, 54.725 |
| Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
| Resolution | 54.550 - 1.300 |
| R-factor | 0.133 |
| Rwork | 0.132 |
| R-free | 0.15300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uzv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.559 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.280 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.160 | 0.110 |
| Number of reflections | 100063 | |
| <I/σ(I)> | 18.6 | 9.5 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | TRIS HCL 0.1M PH8.5, 1.75 M AMMNONIUM SULFATE(THE PA-IIL/GLYCOMIMETIC COMPLEX WAS OBTAINED BY SOAKING METHOD FROM FUCOSE-CONTAINING CRYSTAL) |
