2VTY
Vaccinia virus anti-apoptotic F1L is a novel Bcl-2-like domain swapped dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-10 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 88.261, 88.261, 88.261 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.390 - 2.100 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.20700 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.611 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.550 |
| Number of reflections | 13687 | |
| <I/σ(I)> | 14.4 | 3.5 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 0.9 NACL, 50 MM NA/K PHOSPHATE PH 5.2 |
