2VTV

PhaZ7 depolymerase from Paucimonas lemoignei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	176.383, 46.380, 85.779
Unit cell angles	90.00, 99.78, 90.00

Refinement procedure

Resolution	87.040 - 1.900
R-factor	0.145
Rwork	0.142
R-free	0.18800
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.227
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.060	0.220
Number of reflections	53749
<I/σ(I)>	30.1	6.5
Completeness [%]	99.7	94
Redundancy	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS			MICRODIALYSIS, PH 10.5, NACL 150 MM