2VT7
Native Torpedo californica acetylcholinesterase collected with a cumulated dose of 800000 Gy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2008-05-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.580, 106.060, 150.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.200 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w75 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 73562 | |
| <I/σ(I)> | 15.7 | 4.04 |
| Completeness [%] | 98.1 | 97.6 |
| Redundancy | 7.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 277 | 32% PEG200, 150MM MES, PH6, 4DEG. C., pH 5.8 |
