2VT3
Structure and functional properties of the Bacillus subtilis transcriptional repressor Rex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-17 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.460, 53.520, 86.240 |
| Unit cell angles | 90.00, 125.80, 90.00 |
Refinement procedure
| Resolution | 19.460 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1XCB CHAIN F C-TERMINAL DOMAIN |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.554 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 2.100 | 2.000 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 31994 | |
| <I/σ(I)> | 12.8 | 2.8 |
| Completeness [%] | 99.0 | 93.4 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | RESERVOAR SOLUTION OF 20% W/V PEG6000, 0.2M NH4CL AND 0.1M HEPES PH 7.1 |
