2VSX
Crystal Structure of a Translation Initiation Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-15 |
| Detector | RIGAKU RAXIS-IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.920, 111.360, 111.280 |
| Unit cell angles | 90.00, 99.71, 90.00 |
Refinement procedure
| Resolution | 33.210 - 2.800 |
| R-factor | 0.2535 |
| Rwork | 0.252 |
| R-free | 0.28690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1QDE 1fuk 1hu3 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.594 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 0.700 |
| Number of reflections | 40597 | |
| <I/σ(I)> | 11.5 | 1.6 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 277 | 0.2 M TARTRATE, 20 % PEG 3350, PH 7.2, 4 DEG C, 10 ,MM AMP |
