2VSW
The structure of the rhodanese domain of the human dual specificity phosphatase 16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.451, 69.128, 61.017 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.370 - 2.200 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ouc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.813 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.220 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.120 | 0.690 |
| Number of reflections | 15465 | |
| <I/σ(I)> | 4.94 | 2.63 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 5.09 | 5.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M NANO3, 0.1 M BIS-TRIS-PROPANE PH 8.5, 20% PEG 3350. |
