2VSH
Synthesis of CDP-activated ribitol for teichoic acid precursors in Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 140 |
| Detector technology | CCD |
| Collection date | 2008-04-12 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.000, 93.380, 144.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.930 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vpa |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.278 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.480 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.410 |
| Number of reflections | 36985 | |
| <I/σ(I)> | 16 | 3 |
| Completeness [%] | 99.2 | 96.7 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.75 | 100 MM HEPES PH 6.75 35 % V/W PEG 300 200 MM CACL2 |
