2VSF
Structure of XPD from Thermoplasma acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 78.890, 78.890, 174.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.28700 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.925 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.630 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 13622 | |
| <I/σ(I)> | 8.89 | 1.24 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.98 | 3.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M MGCL2, 10-15% PEG 400, 0.1M SODIUM CITRATE PH5.6 |
