2VS6

K173A, R174A, K177A-trichosanthin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2007-04-23
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	70.025, 95.840, 101.832
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
Rwork	0.191
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tcs
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	MOSFLM
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.990	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.310
Number of reflections	27383
<I/σ(I)>	16.7	5.2
Completeness [%]	99.6	98.2
Redundancy	5.3	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1