2VRZ
Structural analysis of homodimeric staphylococcal aureus EsxA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-02 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.232, 27.636, 64.394 |
| Unit cell angles | 90.00, 105.43, 90.00 |
Refinement procedure
| Resolution | 31.040 - 1.900 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.140 |
| Number of reflections | 13006 | |
| <I/σ(I)> | 21.3 | 2.5 |
| Completeness [%] | 95.5 | 70 |
| Redundancy | 3.6 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1M MES PH 5.5, 80MM ZINC ACETATE, 8% PEG 8000 |
