2VPV
Dimerization Domain of Mif2p
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2005-03-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 106.020, 106.020, 106.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.529 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.430 |
| Number of reflections | 15323 | |
| <I/σ(I)> | 9.6 | 1.5 |
| Completeness [%] | 89.7 | 80.6 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | HANGING DROP VAPOR FUSION FROM 3-4% (W/V) PEG 3000, 100 MM IMIDAZOLE (PH 8.0), AND 70-100 MM LI2SO4 OR NA2SO4 |
