2VPV

Dimerization Domain of Mif2p

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	78
Detector technology	CCD
Collection date	2005-03-15
Detector	ADSC CCD
Spacegroup name	P 21 3
Unit cell lengths	106.020, 106.020, 106.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.21
Rwork	0.208
R-free	0.24200
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.012
RMSD bond angle	1.529
Phasing software	SOLVE
Refinement software	REFMAC (5.4.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.080	0.430
Number of reflections	15323
<I/σ(I)>	9.6	1.5
Completeness [%]	89.7	80.6
Redundancy	1.9	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		HANGING DROP VAPOR FUSION FROM 3-4% (W/V) PEG 3000, 100 MM IMIDAZOLE (PH 8.0), AND 70-100 MM LI2SO4 OR NA2SO4