2VPQ
Crystal structure of biotin carboxylase from S. aureus complexed with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.247, 63.318, 105.142 |
| Unit cell angles | 90.00, 103.82, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.219 |
| Rwork | 0.215 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dv2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.231 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.140 | 0.430 |
| Number of reflections | 55214 | |
| <I/σ(I)> | 8.07 | 1.9 |
| Completeness [%] | 93.8 | 70.1 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M KCL; 20% PEG3350 |
