2VPO
High resolution structure of the periplasmic binding protein TeaA from TeaABC TRAP transporter of Halomonas elongata in complex with hydroxyectoine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.600, 52.100, 63.700 |
| Unit cell angles | 80.80, 85.70, 78.00 |
Refinement procedure
| Resolution | 19.820 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.179 |
| R-free | 0.22500 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.411 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | ARP/wARP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.010 | 0.080 |
| Number of reflections | 53752 | |
| <I/σ(I)> | 8.8 | 2.3 |
| Completeness [%] | 96.2 | 94 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM HEPES PH 7.5, 40 MM MGCL2, 35 % PEG 3350 |
