2VPK
Crystal structure of the BTB domain of human myoneurin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 55.688, 55.688, 126.201 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.317 - 2.000 |
| R-factor | 0.1981 |
| Rwork | 0.194 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2if5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.904 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.140 | 1.100 |
| Number of reflections | 9485 | |
| <I/σ(I)> | 0 | 1.4 |
| Completeness [%] | 98.3 | 98.6 |
| Redundancy | 4.33 | 4.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M NA/KPO4, 0.1 M BIS-TRIS PROPANE PH 6.5, 20% PEG 3350. |
