2VPD
Decoding of methylated histone H3 tail by the Pygo-BCL9 Wnt signaling complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-16 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.720, 100.890, 64.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.670 - 2.770 |
| R-factor | 0.235 |
| Rwork | 0.234 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vpb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.986 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.450 | 2.920 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 30994 | |
| <I/σ(I)> | 22.5 | 4.2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | HANGING-DROP VAPOUR-DIFFUSION METHOD AT 19 CELSIUS. CRYSTALLIZATION CONDITIONS: 1.3M (NH4)2SO4, 100MM TRIS PH6.5, 200MM NACL |
