2VPA
High resolution crystal structure of the antibiotic resistance protein NimA from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.037, 38.951, 59.897 |
| Unit cell angles | 90.00, 114.36, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.142 |
| Rwork | 0.141 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w3o |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.734 |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.090 | 0.610 |
| Number of reflections | 65888 | |
| <I/σ(I)> | 10.2 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.6-0.7 M SODIUM ACETATE AND 0.1 M MES PH 6 OR 0.1 M NA CACODYLATE PH 6.5. |
