2VP1
Fe-FutA2 from Synechocystis PCC6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.340, 51.600, 100.740 |
| Unit cell angles | 90.00, 98.50, 90.00 |
Refinement procedure
| Resolution | 30.210 - 2.700 |
| R-factor | 0.218 |
| Rwork | 0.215 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2voz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.185 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.400 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.290 |
| Number of reflections | 20641 | |
| <I/σ(I)> | 11.9 | 3.8 |
| Completeness [%] | 96.4 | 99.1 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 27% PEG 4000, 0.2M MAGNESIUM CHLORIDE, 0.1M 2-(N-MORPHOLINO)ETHANESULPHONIC ACID (MES) PH 6.5 |
