2VOU
Structure of 2,6-dihydroxypyridine-3-hydroxylase from Arthrobacter nicotinovorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 185.960, 185.960, 104.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.920 - 2.600 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.22200 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.505 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.510 |
| Number of reflections | 64088 | |
| <I/σ(I)> | 15 | 2.4 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 0.1 M SODIUM CACODYLATE PH 6.6, 15% (W/V) PEG 8000, 200 MM MAGNESIUM ACETATE, 20% (V/V) GLYCEROL |
